Floret puccinellia tenuiflora lacks a homologous expression system, as well as genetically modified flowers puccinellia tenuiflora plant materials for easy access to difficult points, grew up spending the initial establishment of the system of puccinellia tenuiflora in suspension culture cells and its biological characteristics of starting, efficient transformation by Agrobacterium with a view to preparing for the receptor material, and develop an effective transformation of the programme, so as to establish an efficient conversion modes. Flowers gained puccinellia tenuiflora cognate expression system will be more stable and accurate genus puccinellia tenuiflora gene function analysis.